IL18BP ELISA 试剂盒
Quick Overview for IL18BP ELISA 试剂盒 (ABIN6810505)
抗原
See all IL18BP ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 22 pg/mL
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原理
- Mouse IL-18 BPc ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair recognizes Mouse IL-18 BPc.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 18 Binding Protein (IL18BP) ELISA Kit
IL18BP 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 AA 31-194 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 94-6000 pg/mL Cell Lysate, Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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试剂准备
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Recommended Dilution for serum and plasma samples4 fold
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限制
- 仅限研究用
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有效期
- 6 months
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- IL18BP (Interleukin 18 Binding Protein (IL18BP))
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别名
- IL-18 BPc
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UniProt
- Q9Z0M9
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